Charge-induced patchy attractions between proteins.
J Phys Chem B
; 119(2): 503-8, 2015 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-25494398
ABSTRACT
Static light scattering (SLS) combined with structure-based Monte Carlo (MC) simulations provide new insights into mechanisms behind anisotropic, attractive protein interactions. A nonmonotonic behavior of the osmotic second virial coefficient as a function of ionic strength is here shown to originate from a few charged amino acids forming an electrostatic attractive patch, highly directional and complementary. Together with Coulombic repulsion, this attractive patch results in two counteracting electrostatic contributions to the interaction free energy which, by operating over different length scales, is manifested in a subtle, salt-induced minimum in the second virial coefficient as observed in both experiment and simulations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Método de Monte Carlo
/
Eletricidade Estática
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Revista:
J Phys Chem B
Assunto da revista:
QUIMICA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Suécia