An enzyme-linked immunosorbent assay for detection of pyrene and related polycyclic aromatic hydrocarbons.
Anal Biochem
; 473: 1-6, 2015 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-25524617
ABSTRACT
Polycyclic aromatic hydrocarbons (PAHs) can form DNA-binding compounds that show genotoxicity and carcinogenicity. Pyrene, as a PAH, was covalently linked to carrier protein bovine serum albumin and ovalbumin. A monoclonal antibody (McAb) was produced that showed high cross-reactivity values with chrysene (169.73%), benzo[a]pyrene (693.34%), benzo[a]anthracene (16.36%), and indeno[1,2,3-cd]pyrene (40.96%) and showed no significant cross-reactivity values with other homologues (<0.1%). A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of pyrene and some homologues in water samples. The detection limit of the assay was 65.08 pg ml(-1). The average recoveries of PAHs from tap water, lake water, and mineral water were 99.13, 99.74, and 99.19%, respectively, indicating that matrices of water samples do not interfere with the assay. The results demonstrated that the developed ELISA seems to be a potential method for monitoring of pyrene and some homologous PAHs in water samples.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pirenos
/
Ensaio de Imunoadsorção Enzimática
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2015
Tipo de documento:
Article