Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats.
Food Chem
; 174: 37-43, 2015 May 01.
Article
em En
| MEDLINE
| ID: mdl-25529649
ABSTRACT
The aim of this study was to investigate antihypertensive effect of bioactive peptides from skate (Okamejei kenojei) skin gelatin. The Alcalase/protease gelatin hydrolysate below 1 kDa (SAP) exhibited the highest angiotensin-I converting enzyme (ACE) inhibition compared to other hydrolysates. SAP can decrease systolic blood pressure significantly in spontaneously hypertensive rats. SAP inhibited vasoconstriction via PPAR-γ expression, activation and phosphorylation of eNOS in lungs. Moreover, the expression levels of endothelin-1, RhoA, α-smooth muscle actin, cleaved caspase 3 and MAPK were decreased by SAP in lungs. Vascularity, muscularization and cellular proliferation in lungs were detected by immunohistochemical staining. Finally, two purified peptides (LGPLGHQ, 720Da and MVGSAPGVL, 829Da) showed potent ACE inhibition with IC50 values of 4.22 and 3.09 µM, respectively. These results indicate that bioactive peptides isolated from skate skin gelatin may serve as candidates against hypertension and could be used as functional food ingredients.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Pele
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Rajidae
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Inibidores da Enzima Conversora de Angiotensina
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Proteínas de Peixes
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Gelatina
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Hipertensão
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Anti-Hipertensivos
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2015
Tipo de documento:
Article