Chirality-assisted ring-like aggregation of aß(1-40) at liquid-solid interfaces: a stereoselective two-step assembly process.

ABSTRACT

Molecular chirality is introduced at liquid-solid interfaces. A ring-like aggregation of amyloid Aß(1-40) on N-isobutyryl-L-cysteine (L-NIBC)-modified gold substrate occurs at low Aß(1-40) concentration, while D-NIBC modification only results in rod-like aggregation. Utilizing atomic force microscope controlled tip-enhanced Raman scattering, we directly observe the secondary structure information for Aß(1-40) assembly in situ at the nanoscale. D- or L-NIBC on the surface can guide parallel or nonparallel alignment of ß-hairpins through a two-step process based on electrostatic-interaction-enhanced adsorption and subsequent stereoselective recognition. Possible electrostatic interaction sites (R5 and K16) and a chiral recognition site (H14) of Aß(1-40) are proposed, which may provide insight into the understanding of this effect.