The E6 and E7 genes of HPV-18 are sufficient for inducing two-stage in vitro transformation of human keratinocytes.
Oncogene
; 4(12): 1529-32, 1989 Dec.
Article
em En
| MEDLINE
| ID: mdl-2556677
ABSTRACT
Using a recently described keratinocyte assay, we demonstrate that HPV-18 DNA induces two progressive steps in cellular transformation (a large cell and a small cell stage). Both steps of this keratinocyte transformation can be achieved with a subgenomic fragment containing only the HPV-18 regulatory region and E6/E7 genes. Similar to cell lines transformed by the complete HPV-18 genome, keratinocytes transformed by the HPV-18 E6/E7 genes express the major early viral protein (E7) but are non-tumorigenic in nude mice. Interestingly, HPV-18 DNA was noted to be 5 times more efficient than HPV-16 DNA for in vitro keratinocyte transformation, regardless of the method of DNA transfection.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
DNA Viral
/
Queratinócitos
/
Transformação Celular Neoplásica
/
Genes Virais
Limite:
Humans
/
Male
Idioma:
En
Revista:
Oncogene
Assunto da revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Ano de publicação:
1989
Tipo de documento:
Article