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Biosynthesis of copper nanoparticles and its effect on actively dividing cells of mitosis in Allium cepa.
Nagaonkar, Dipali; Shende, Sudhir; Rai, Mahendra.
Afiliação
  • Nagaonkar D; Nanobiotechnology Laboratory, Dept. of Biotechnology, S.G.B. Amravati University, Amravati, 444 602, Maharashtra, India.
Biotechnol Prog ; 31(2): 557-65, 2015.
Article em En | MEDLINE | ID: mdl-25607830
ABSTRACT
Nanobiotechnological application of copper nanoparticles has paved the way for advancement in agriculture owing to its bactericidal and fungicidal activities. Recently, researchers have focussed on bioinspired synthesis of copper nanoparticles as a viable alternative to existing physicochemical techniques. For the commercialization of nanocopper, the toxicity evaluation is a major issue. In this context, Citrus medica (L.) fruit extract-mediated copper nanoparticles were synthesized and its different concentrations (10, 20, 40, 60, 80, and 100 µg mL(-1) ) were evaluated for its effect on actively dividing cells of Allium cepa. The study clearly revealed that copper nanoparticles increased mitotic index up to the concentration of 20 µg mL(-1) . In addition, a gradual decline in mitotic index and increase in abnormality index was observed as the concentration of copper nanoparticles and treatment duration were increased. Aberrations in chromosomal behavior such as sticky and disturbed chromosomes in metaphase and anaphase, c-metaphase, bridges, laggard, disturbed telophase, and vacuolated nucleus were also observed.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cobre / Cebolas / Proliferação de Células / Nanopartículas Metálicas / Mitose Idioma: En Revista: Biotechnol Prog Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cobre / Cebolas / Proliferação de Células / Nanopartículas Metálicas / Mitose Idioma: En Revista: Biotechnol Prog Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Índia