Ribose-seq: global mapping of ribonucleotides embedded in genomic DNA.
Nat Methods
; 12(3): 251-7, 3 p following 257, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25622106
ABSTRACT
Abundant ribonucleotide incorporation in DNA during replication and repair has profound consequences for genome stability, but the global distribution of ribonucleotide incorporation is unknown. We developed ribose-seq, a method for capturing unique products generated by alkaline cleavage of DNA at embedded ribonucleotides. High-throughput sequencing of these fragments in DNA from the yeast Saccharomyces cerevisiae revealed widespread ribonucleotide distribution, with a strong preference for cytidine and guanosine, and identified hotspots of ribonucleotide incorporation in nuclear and mitochondrial DNA. Ribonucleotides were primarily incorporated on the newly synthesized leading strand of nuclear DNA and were present upstream of (G+C)-rich tracts in the mitochondrial genome. Ribose-seq is a powerful tool for the systematic profiling of ribonucleotide incorporation in genomic DNA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribonucleotídeos
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Saccharomyces cerevisiae
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Técnicas Genéticas
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos