Transcriptome wide annotation of eukaryotic RNase III reactivity and degradation signals.
PLoS Genet
; 11(2): e1005000, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25680180
ABSTRACT
Detection and validation of the RNA degradation signals controlling transcriptome stability are essential steps for understanding how cells regulate gene expression. Here we present complete genomic and biochemical annotations of the signals required for RNA degradation by the dsRNA specific ribonuclease III (Rnt1p) and examine its impact on transcriptome expression. Rnt1p cleavage signals are randomly distributed in the yeast genome, and encompass a wide variety of sequences, indicating that transcriptome stability is not determined by the recurrence of a fixed cleavage motif. Instead, RNA reactivity is defined by the sequence and structural context in which the cleavage sites are located. Reactive signals are often associated with transiently expressed genes, and their impact on RNA expression is linked to growth conditions. Together, the data suggest that Rnt1p reactivity is triggered by malleable RNA degradation signals that permit dynamic response to changes in growth conditions.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Estabilidade de RNA
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Proteínas de Saccharomyces cerevisiae
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Ribonuclease III
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Transcriptoma
Idioma:
En
Revista:
PLoS Genet
Assunto da revista:
GENETICA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Canadá