Oncogenic miR-23a in Pancreatic Ductal Adenocarcinogenesis Via Inhibiting APAF1.
Dig Dis Sci
; 60(7): 2000-8, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25701323
ABSTRACT
BACKGROUND:
miR-23a, which participates in invasion of pancreatic ductal adenocarcinoma cells into the mesothelial barrier, is a critical regulator in many cancers. It, however, is still unknown whether miR-23a regulates pancreatic cell proliferation and apoptosis or not.AIMS:
We sought to investigate the role of miR-23a in regulation of pancreatic cell proliferation and apoptosis.METHODS:
miRNA, mRNA, and protein expressions were determined by qRT-PCR and Western blot, respectively. Dual-luciferase reporter assay was used in detection for binding ability of miR-23a to APAF1. Ectopic miR-23a and APAF 1 were introduced to pancreatic cells, and their roles in proliferation and apoptosis were detected by MTT, colony formation, and apoptosis assays, respectively.RESULTS:
Up-regulation of miR-23a and down-regulation of APAF 1 were found in pancreatic ductal cancer, respectively. miR-23a significantly inhibited the luciferase activity by targeting APAF 1 3'UTR. Ectopic miR-23a significantly suppressed the APAF 1 gene expression in pancreatic cancer cells. Similar to siAPAF1, miR-23a significantly promoted pancreatic cancer cell proliferation and repressed apoptosis. Furthermore, miR-23a inhibitor and exogenous APAF 1 could recover the effects.CONCLUSIONS:
It is suggested that miR-23a, acting as an oncogenic regulator by directly targeting APAF 1 in pancreatic cancer, is a useful potential biomarker in diagnosis and treatment of pancreatic cancer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ductos Pancreáticos
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Neoplasias Pancreáticas
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Adenocarcinoma
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MicroRNAs
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Fator Apoptótico 1 Ativador de Proteases
Limite:
Humans
Idioma:
En
Revista:
Dig Dis Sci
Ano de publicação:
2015
Tipo de documento:
Article