Aequorin-based luminescence imaging reveals differential calcium signalling responses to salt and reactive oxygen species in rice roots.
J Exp Bot
; 66(9): 2535-45, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25754405
ABSTRACT
It is well established that both salt and reactive oxygen species (ROS) stresses are able to increase the concentration of cytosolic free Ca(2+) ([Ca(2+)]i), which is caused by the flux of calcium (Ca(2+)). However, the differences between these two processes are largely unknown. Here, we introduced recombinant aequorin into rice (Oryza sativa) and examined the change in [Ca(2+)]i in response to salt and ROS stresses. The transgenic rice harbouring aequorin showed strong luminescence in roots when treated with exogenous Ca(2+). Considering the histological differences in roots between rice and Arabidopsis, we reappraised the discharging solution, and suggested that the percentage of ethanol should be 25%. Different concentrations of NaCl induced immediate [Ca(2+)]i spikes with the same durations and phases. In contrast, H2O2 induced delayed [Ca(2+)]i spikes with different peaks according to the concentrations of H2O2. According to the Ca(2+) inhibitor research, we also showed that the sources of Ca(2+) induced by NaCl and H2O2 are different. Furthermore, we evaluated the contribution of [Ca(2+)]i responses in the NaCl- and H2O2-induced gene expressions respectively, and present a Ca(2+)- and H2O2-mediated molecular signalling model for the initial response to NaCl in rice.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Cloreto de Sódio
/
Espécies Reativas de Oxigênio
/
Regulação da Expressão Gênica de Plantas
/
Sinalização do Cálcio
Idioma:
En
Revista:
J Exp Bot
Assunto da revista:
BOTANICA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China