Detection of exosomal prions in blood by immunochemistry techniques.
J Gen Virol
; 96(Pt 7): 1969-74, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25805411
ABSTRACT
In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in blood remains elusive. We developed a novel method for the detection of PrPTSE in blood of prion-infected rodents based on the finding that PrPTSE is associated with plasma exosomes. However, further purification of the exosomes on a sucrose gradient was necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its detection by immunochemistry. Finally, we report that about 20% of plasma infectivity is associated with exosomes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Príons
/
Exossomos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Itália