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Associations between Gut Microbial Colonization in Early Life and Respiratory Outcomes in Cystic Fibrosis.
Hoen, Anne G; Li, Jing; Moulton, Lisa A; O'Toole, George A; Housman, Molly L; Koestler, Devin C; Guill, Margaret F; Moore, Jason H; Hibberd, Patricia L; Morrison, Hilary G; Sogin, Mitchell L; Karagas, Margaret R; Madan, Juliette C.
Afiliação
  • Hoen AG; Computational Genetics Laboratory, Institute for Quantitative Biomedical Sciences, Geisel School of Medicine at Dartmouth, Dartmouth College, Hanover, NH; Department of Epidemiology, Geisel School of Medicine at Dartmouth, Dartmouth College, Hanover, NH.
  • Li J; Computational Genetics Laboratory, Institute for Quantitative Biomedical Sciences, Geisel School of Medicine at Dartmouth, Dartmouth College, Hanover, NH.
  • Moulton LA; Division of Allergy and Pediatric Pulmonology, Department of Pediatrics, Dartmouth-Hitchcock Medical Center, Lebanon, NH.
  • O'Toole GA; Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH.
  • Housman ML; Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH.
  • Koestler DC; Department of Biostatistics, University of Kansas Medical Center, Kansas City, KS.
  • Guill MF; Division of Allergy and Pediatric Pulmonology, Department of Pediatrics, Dartmouth-Hitchcock Medical Center, Lebanon, NH.
  • Moore JH; Computational Genetics Laboratory, Institute for Quantitative Biomedical Sciences, Geisel School of Medicine at Dartmouth, Dartmouth College, Hanover, NH.
  • Hibberd PL; Division of Global Health, Department of Pediatrics, Massachusetts General Hospital, Boston, MA.
  • Morrison HG; Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA.
  • Sogin ML; Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA.
  • Karagas MR; Department of Epidemiology, Geisel School of Medicine at Dartmouth, Dartmouth College, Hanover, NH.
  • Madan JC; Division of Neonatology, Department of Pediatrics, Dartmouth-Hitchcock Medical Center, Lebanon, NH. Electronic address: Juliette.C.Madan@Dartmouth.Edu.
J Pediatr ; 167(1): 138-47.e1-3, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25818499
OBJECTIVE: To examine patterns of microbial colonization of the respiratory and intestinal tracts in early life in infants with cystic fibrosis (CF) and their associations with breastfeeding and clinical outcomes. STUDY DESIGN: A comprehensive, prospective longitudinal analysis of the upper respiratory and intestinal microbiota in a cohort of infants and young children with CF followed from birth was performed. Genus-level microbial community composition was characterized using 16S-targeted pyrosequencing, and relationships with exposures and outcomes were assessed using linear mixed-effects models, time-to-event analysis, and principal components analysis. RESULTS: Sequencing of 120 samples from 13 subjects collected from birth to 34 months revealed relationships between breastfeeding, microbial diversity in the respiratory and intestinal tracts, and the timing of onset of respiratory complications, including exacerbations and colonization with Pseudomonas aeruginosa. Fluctuations in the abundance of specific bacterial taxa preceded clinical outcomes, including a significant decrease in bacteria of the genus Parabacteroides within the intestinal tract prior to the onset of chronic P aeruginosa colonization. Specific assemblages of bacteria in intestinal samples, but not respiratory samples, were associated with CF exacerbation in early life, indicating that the intestinal microbiome may play a role in lung health. CONCLUSIONS: Our findings relating breastfeeding to respiratory outcomes, gut diversity to prolonged periods of health, and specific bacterial communities in the gut prior to respiratory complications in CF highlight a connection between the intestinal microbiome and health and point to potential opportunities for antibiotic or probiotic interventions. Further studies in larger cohorts validating these findings are needed.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistema Respiratório / Fibrose Cística / Microbiota / Intestinos Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Child, preschool / Humans / Infant / Newborn Idioma: En Revista: J Pediatr Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistema Respiratório / Fibrose Cística / Microbiota / Intestinos Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Child, preschool / Humans / Infant / Newborn Idioma: En Revista: J Pediatr Ano de publicação: 2015 Tipo de documento: Article