Chemical composition and antioxidant activity of ultrasound-assisted extract of the endemic plant Haberlea rhodopensis Friv.
J Food Sci Technol
; 52(4): 2469-73, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25829635
ABSTRACT
The antioxidant capacity of extracts from leaves of Haberlea rhodopensis Friv. obtained by a highly efficient and simple method of the ultrasound-assisted extraction was investigated. The total polyphenolic content was determined spectrophotometrically using the Folin - Ciocalteu's phenol reagent and the polyphenols composition was analyzed by HPLC method. Antioxidant activity of the extract was evaluated as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azinobis-3 ethyl benxothiazoline-6-sulphonic acid (ABTS) cation decolorization activity assays and the ferric reducing/antioxidant power method (FRAP). The total phenolics of the extract were determined to be 148.71 ± 5.33 mg GAE on a dry weight basis. The HPLC analysis showed a large number of flavonoids and phenolic acids present in the ultrasound ethanol extract of H. rhodopensis. Results also revealed that the major flavonoids were luteolin (2730.18 µg/g DW), hesperidin (928.56 µg/g DW) and kaempferol (578.52 µg/g DW), whereas the most abundant phenolic acids identified in the extract were ferulic acid (630.48 µg/g DW) and sinapic acid (580.80 µg/g DW). DPPH(â¢) and ABTS(â¢+) values were 0.803 ± 0.007 and 0.769 ± 0.040 mmol TE/g DW, respectively and FRAP values were 1.362 ± 0.05 mmol TE/g DW.
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MEDLINE
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En
Revista:
J Food Sci Technol
Ano de publicação:
2015
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Article