[Comparative study on pharmacokinetics and tissue distribution of cis- and trans-2,3,5,4'-Tetrahydroxystilbene-2-O-beta-D-glucosides in mice].
Zhong Yao Cai
; 37(9): 1627-32, 2014 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-25857165
ABSTRACT
OBJECTIVE:
To develop a method for simultaneous determination of cis-2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (cis-THSG) and trans-2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (trans-THSG) in mice and comparative study of cis-THSG and trans-THSG in pharmacokinetic and tissue distribution.METHODS:
Analyses was performed using a Diamonsil C,18 (250 mm x 4.6 mm, 5 microm) column with acetonitrile-methanol-0.1% acetic acid (117 82) as the mobile phase at a flow rate of 1.0 mL/min. Polydatin was took as the internal standard. The detection wavelength was set at 285 and 320 nm.RESULTS:
Intra-day and inter-day RSD were less than 9.47%. The recoveries and stabilities( RE) were ranged from -9.69% to 4.18% and from -9.49% to 1.33%, respectively. The pharmacokinetic parameters showed that the elimination half-life of cis-THSG was longer than that of trans-THSG and the biological availability of cis-THSG was higher than that of trans-THSG. Both cis-THSG and trans-THSG were widely distributed in the tissues. At the same dosage,the tissue concentrations of trans-THSG were greater than those of cis-THSG.CONCLUSION:
The method is specific,sensitive, simple, rapid and suitable for simultaneous determination of cis-THSG and trans-THSG in mice. The results of pharmacokinetic and tissue distribution indicated that there was significant difference between cis-THSG and trans-THSG in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Estilbenos
/
Glucosídeos
Limite:
Animals
Idioma:
Zh
Revista:
Zhong Yao Cai
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2014
Tipo de documento:
Article