Kaiso mainly locates in the nucleus in vivo and binds to methylated, but not hydroxymethylated DNA.
Chin J Cancer Res
; 27(2): 148-55, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25937776
ABSTRACT
OBJECTIVE:
Kaiso is upregulated in many cancers and proposed to bind with both methylated- and unmethylated-DNA in the nucleus as a transcriptional repressor. The objective is to define its subcellular localization in vivo and exact binding DNA sequences in cells.METHODS:
Compartmentalization of exogenous Kaiso in cells was tracked with enhanced green fluorescence protein (EGFP) tag. The endogenous Kaiso expression in gastric carcinoma tissue was examined with immunohistochemical staining. Kaiso-DNA binding was tested using electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation assay (ChIP).RESULTS:
Kaiso mainly localized in the nucleus of cancer and stromal cells in vivo, but remained in the cytoplasm of cultured cells. Most importantly, nuclear Kaiso can bind with the methylated-CGCG-containing sequence in the CDKN2A promoter, but not with the hydroxymethylated-CGCG sequence in HCT116 cells.CONCLUSIONS:
Kaiso locates mainly in the nucleus in vivo where it binds with the methylated-CGCG sequences, but does not bind with the hydroxymethylated-CGCG sequences.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Chin J Cancer Res
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China