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Towards early detection of cervical cancer: Fractal dimension of AFM images of human cervical epithelial cells at different stages of progression to cancer.
Guz, Nataliia V; Dokukin, Maxim E; Woodworth, Craig D; Cardin, Andrew; Sokolov, Igor.
Afiliação
  • Guz NV; Department of Physics, Clarkson University, Potsdam, NY, USA.
  • Dokukin ME; Department of Mechanical Engineering, Tufts University, Medford, MA, USA.
  • Woodworth CD; Department of Biology, Clarkson University, Potsdam, NY, USA.
  • Cardin A; Department of Physics, Clarkson University, Potsdam, NY, USA.
  • Sokolov I; Department of Mechanical Engineering, Tufts University, Medford, MA, USA; Department of Biomedical Engineering, Tufts University, Medford, MA, USA; Department of Physics, Tufts University, Medford, MA, USA. Electronic address: igor.sokolov@tufts.edu.
Nanomedicine ; 11(7): 1667-75, 2015 Oct.
Article em En | MEDLINE | ID: mdl-25959926
ABSTRACT
We used AFM HarmoniX modality to analyse the surface of individual human cervical epithelial cells at three stages of progression to cancer, normal, immortal (pre-malignant) and carcinoma cells. Primary cells from 6 normal strains, 6 cancer, and 6 immortalized lines (derived by plasmid DNA-HPV-16 transfection of cells from 6 healthy individuals) were tested. This cell model allowed for good control of the cell phenotype down to the single cell level, which is impractical to attain in clinical screening tests (ex-vivo). AFM maps of physical (nonspecific) adhesion are collected on fixed dried cells. We show that a surface parameter called fractal dimension can be used to segregate normal from both immortal pre-malignant and malignant cells with sensitivity and specificity of more than 99%. The reported method of analysis can be directly applied to cells collected in liquid cytology screening tests and identified as abnormal with regular optical methods to increase sensitivity. FROM THE CLINICAL EDITOR Despite cervical smear screening, sometimes it is very difficult to differentiate cancers cells from pre-malignant cells. By using AFM to analyze the surface properties of human cervical epithelial cells, the authors were able to accurately identify normal from abnormal cells. This method could augment existing protocols to increase diagnostic accuracy.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias do Colo do Útero / Microscopia de Força Atômica / Células Epiteliais / Detecção Precoce de Câncer Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies / Screening_studies Limite: Female / Humans Idioma: En Revista: Nanomedicine Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias do Colo do Útero / Microscopia de Força Atômica / Células Epiteliais / Detecção Precoce de Câncer Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies / Screening_studies Limite: Female / Humans Idioma: En Revista: Nanomedicine Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos