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Targeted Mutagenesis in Zebrafish Using CRISPR RNA-Guided Nucleases.
Hwang, Woong Y; Fu, Yanfang; Reyon, Deepak; Gonzales, Andrew P W; Joung, J Keith; Yeh, Jing-Ruey Joanna.
Afiliação
  • Hwang WY; Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA, 02129, USA.
Methods Mol Biol ; 1311: 317-34, 2015.
Article em En | MEDLINE | ID: mdl-25981483
In recent years, the zebrafish has become a critical contributor to various areas of biomedical research, advancing our fundamental understanding of biomedicine and helping discover candidate therapeutics for human diseases. Nevertheless, to further extend the power of this important model organism requires a robust and simple-to-use genome editing platform that will enable targeted gene knockouts and introduction of specific mutations identified in human diseases into the zebrafish genome. We describe here protocols for creating insertion or deletion (indel) mutations or precise sequence modifications in zebrafish genes using customizable CRISPR-Cas9 RNA-guided nucleases (RGNs). These methods can be easily implemented in any lab and may also potentially be extended for use in other organisms.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peixe-Zebra / RNA / Engenharia Genética / Mutagênese / Desoxirribonucleases / Proteínas Associadas a CRISPR / Sistemas CRISPR-Cas Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peixe-Zebra / RNA / Engenharia Genética / Mutagênese / Desoxirribonucleases / Proteínas Associadas a CRISPR / Sistemas CRISPR-Cas Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos