Arsenic Trioxide Inhibits Cell Growth and Invasion via Down- Regulation of Skp2 in Pancreatic Cancer Cells.
Asian Pac J Cancer Prev
; 16(9): 3805-10, 2015.
Article
em En
| MEDLINE
| ID: mdl-25987041
ABSTRACT
Arsenic trioxide (ATO) has been found to exert anti-cancer activity in various human malignancies. However, the molecular mechanisms by which ATO inhibits tumorigenesis are not fully elucidated. In the current study, we explored the molecular basis of ATO-mediated tumor growth inhibition in pancreatic cancer cells. We used multiple approaches such as MTT assay, wound healing assay, Transwell invasion assay, annexin V-FITC, cell cycle analysis, RT-PCR and Western blotting to achieve our goal. We found that ATO treatment effectively caused cell growth inhibition, suppressed clonogenic potential and induced G2-M cell cycle arrest and apoptosis in pancreatic cancer cells. Moreover, we observed a significant down-regulation of Skp2 after treatment with ATO. Furthermore, we revealed that ATO regulated Skp2 downstream genes such as FOXO1 and p53. These findings demonstrate that inhibition of Skp2 could be a novel strategy for the treatment of pancreatic cancer by ATO.
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Base de dados:
MEDLINE
Assunto principal:
Óxidos
/
Neoplasias Pancreáticas
/
Arsenicais
/
Regulação Neoplásica da Expressão Gênica
/
Movimento Celular
/
Proteínas Quinases Associadas a Fase S
/
Proliferação de Células
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Asian Pac J Cancer Prev
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2015
Tipo de documento:
Article