CD4(+) lymphocytes improve venous blood flow in experimental arteriovenous fistulae.
Surgery
; 158(2): 529-36, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-25999254
ABSTRACT
BACKGROUND:
The role of immune cells in arteriovenous fistulae (AVF) maturation is poorly understood and has received, until quite recently, little attention. This study examines the function of T lymphocytes in AVF vascular remodeling.METHODS:
Experimental fistulae were created in athymic rnu nude rats lacking mature T lymphocytes and euthymic control animals by anastomosing the left superior epigastric vein to the nearby femoral artery. Blood flow rates, wall morphology, and histologic changes were assessed in AVF 21 days after creation. The effect of CD4(+) lymphocytes on AVF maturation in athymic animals was analyzed by adoptive transfer of cells after fistula creation.RESULTS:
The absence of T lymphocytes compromised blood flow in experimental fistulae. Histopathologic inspection of AVF from athymic rats revealed that T-cell immunodeficiency negatively affected venous vascular remodeling, as evidenced by a reduced lumen, a thick muscular layer, and a low number of inflammatory cells compared with control animals. Adoptive transfer of CD4(+) lymphocytes from euthymic rats into athymic animals after fistula creation improved blood flow and reduced intima-media thickness.CONCLUSION:
These results point at the protective role of CD4(+) lymphocytes in the remodeling of the AVF vascular wall.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Derivação Arteriovenosa Cirúrgica
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Linfócitos T CD4-Positivos
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Artéria Femoral
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Remodelação Vascular
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Surgery
Ano de publicação:
2015
Tipo de documento:
Article