Reduced Fluorescence versus Forward Scatter Time-of-Flight and Increased Peak versus Integral Fluorescence Ratios Indicate Receptor Clustering in Flow Cytometry.

Fürnrohr, Barbara G; Stein, Merle; Rhodes, Benjamin; Chana, Prabhjoat S; Schett, Georg; Vyse, Timothy J; Herrmann, Martin; Mielenz, Dirk

Fürnrohr, Barbara G; Stein, Merle; Rhodes, Benjamin; Chana, Prabhjoat S; Schett, Georg; Vyse, Timothy J; Herrmann, Martin; Mielenz, Dirk.

Afiliação

Fürnrohr BG; Division of Molecular Immunology, Department of Internal Medicine 3, University of Erlangen-Nuremberg, Nikolaus Fiebiger Centre, 91054 Erlangen, Germany; Division of Genetics and Molecular Medicine, King's College London, Guy's Hospital, London SE1 9RT, United Kingdom; Division of Immunology, Infect
Stein M; Division of Molecular Immunology, Department of Internal Medicine 3, University of Erlangen-Nuremberg, Nikolaus Fiebiger Centre, 91054 Erlangen, Germany;
Rhodes B; Division of Genetics and Molecular Medicine, King's College London, Guy's Hospital, London SE1 9RT, United Kingdom; Division of Immunology, Infection and Inflammatory Disease, King's College London, Guy's Hospital, London SE1 9RT, United Kingdom; Department of Rheumatology, University Hospitals Birm
Chana PS; Flow Cytometry Core Facility, National Institute for Health Research, Guy's and St. Thomas' National Health Service Foundation Trust/King's College London Comprehensive Biomedical Research Centre, Guy's Hospital, London SE1 9RT, United Kingdom;
Schett G; Department of Internal Medicine 3, University of Erlangen-Nuremberg, 91054 Erlangen, Germany; and Institute for Clinical Immunology, Department of Internal Medicine 3, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
Vyse TJ; Division of Genetics and Molecular Medicine, King's College London, Guy's Hospital, London SE1 9RT, United Kingdom; Division of Immunology, Infection and Inflammatory Disease, King's College London, Guy's Hospital, London SE1 9RT, United Kingdom;
Herrmann M; Department of Internal Medicine 3, University of Erlangen-Nuremberg, 91054 Erlangen, Germany; and Institute for Clinical Immunology, Department of Internal Medicine 3, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
Mielenz D; Division of Molecular Immunology, Department of Internal Medicine 3, University of Erlangen-Nuremberg, Nikolaus Fiebiger Centre, 91054 Erlangen, Germany; dmielenz@molmed.uni-erlangen.de dmielenz@gmx.de.

J Immunol ; 195(1): 377-85, 2015 Jul 01.

Article em En | MEDLINE | ID: mdl-26026066

Assuntos

Linfócitos B/metabolismo; Citometria de Fluxo/métodos; Antígeno de Macrófago 1/química; Neutrófilos/metabolismo; Receptores de Antígenos de Linfócitos B/química; Citoesqueleto de Actina/química; Citoesqueleto de Actina/imunologia; Animais; Linfócitos B/imunologia; Linfócitos B/ultraestrutura; Carbocianinas/química; Separação Celular; Fluorescência; Corantes Fluorescentes/química; Ensaios de Triagem em Larga Escala; Humanos; Fragmentos Fab das Imunoglobulinas/química; Fragmentos Fab das Imunoglobulinas/imunologia; Antígeno de Macrófago 1/imunologia; Camundongos; Camundongos Endogâmicos C57BL; Neutrófilos/imunologia; Neutrófilos/ultraestrutura; Cultura Primária de Células; Transporte Proteico; Receptores de Antígenos de Linfócitos B/imunologia; Coloração e Rotulagem/métodos

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos B / Receptores de Antígenos de Linfócitos B / Antígeno de Macrófago 1 / Citometria de Fluxo / Neutrófilos Limite: Animals / Humans Idioma: En Revista: J Immunol Ano de publicação: 2015 Tipo de documento: Article

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