A Fluorescence-Based Assay for Proteinuria Screening in Larval Zebrafish (Danio rerio).
Zebrafish
; 12(5): 372-6, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26125680
ABSTRACT
Analysis of genes compromising the glomerular filtration barrier in rodent models using transgenic or knockdown approaches is time- and resource-consuming and often leads to unsatisfactory results. Therefore, it would be beneficial to have a selection tool indicating that your gene of interest is in fact associated with proteinuria. Zebrafish (Danio rerio) is a rapid screening tool to study effects in glomerular filtration barrier integrity after genetic manipulation. We use either injection of high-molecular-weight dextrans or a transgenic fluorescent fish line [Tg(l-fabpDBPEGFP)] expressing a vitamin D-binding protein fused with eGFP for indirect detection of proteinuria. A loss of high-molecular-weight proteins from the circulation of the fish into the urine can be identified by monitoring fluorescence intensity in the zebrafish eye. Paired with an optimized analysis method, this assay provides an effective screening solution to detect filtration barrier damage with proteinuria before moving to a mammalian system.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteinúria
/
Peixe-Zebra
/
Dextranos
/
Modelos Animais
/
Olho
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
Zebrafish
Assunto da revista:
BIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Alemanha