Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium.
Biochemistry
; 54(31): 4863-76, 2015 Aug 11.
Article
em En
| MEDLINE
| ID: mdl-26174911
ABSTRACT
Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu(24) and Trp(87) to yield the mature (circular) enterocin NKR-5-3B.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bacteriocinas
/
Enterococcus faecium
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Japão