A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Campylobacter jejuni, C. fetus, C. coli, C. upsaliensis, C. hyointestinalis, and C. lari.
Jpn J Infect Dis
; 69(3): 256-8, 2016 May 20.
Article
em En
| MEDLINE
| ID: mdl-26255737
ABSTRACT
In this study, we devised a multiplex PCR assay based on the gene of cytolethal distending toxin (cdt) B subunit to simultaneously detect and discriminate Campylobacter jejuni, C. fetus, C. coli, C. upsaliensis, C. hyointestinalis, and C. lari. Species-specific PCR products were successfully obtained from all 38 C. jejuni, 12 C. fetus, 39 C. coli, 22 C. upsaliensis, 24 C. hyointestinalis, and 7 C. lari strains tested. On the other hand, no specific PCR products were obtained from other campylobacters and bacterial species tested (41 strains in total). The proposed multiplex PCR assay is a valuable tool for detection and descrimination of 6 major Campylobacter species, that are associated with gastrointestinal diseases in humans.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Campylobacter fetus
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Infecções por Campylobacter
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Campylobacter jejuni
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Campylobacter coli
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Campylobacter hyointestinalis
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Campylobacter lari
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Campylobacter upsaliensis
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Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Jpn J Infect Dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
Ano de publicação:
2016
Tipo de documento:
Article