The Potential of High-throughput Metagenomic Sequencing of Aquatic Bacterial Communities to Estimate the Postmortem Submersion Interval.
J Forensic Sci
; 60(6): 1500-10, 2015 11.
Article
em En
| MEDLINE
| ID: mdl-26294275
ABSTRACT
Human remains can be discovered in freshwater or marine ecosystems, circumstances where insects and other invertebrates have infrequently been used for understanding the time of postmortem submersion. In this study, the identification and succession of epinecrotic bacterial communities on vertebrate remains were described during decomposition in a temperate headwater stream during two seasons (summer and winter). Bacterial communities were characterized with 454 pyrosequencing and analyzed at phyletic and generic taxonomic resolutions. There was a significant increase in genera richness over decomposition during both seasons. Additionally, multivariate statistical modeling revealed significant differences in bacterial communities between seasons at both taxonomic resolutions and siginificant genera differences among sampling days within each season, suggesting a succession of these communities. These data are the first to describe aquatic bacterial succession using high-throughput metagenomic sequencing on vertebrate remains submerged in a freshwater habitat, and provide initial evidence for their potential use in forensic investigations.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mudanças Depois da Morte
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Bactérias
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Sequenciamento de Nucleotídeos em Larga Escala
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Imersão
Limite:
Animals
Idioma:
En
Revista:
J Forensic Sci
Ano de publicação:
2015
Tipo de documento:
Article