GATA-1 directly regulates Nanog in mouse embryonic stem cells.
Biochem Biophys Res Commun
; 465(3): 575-9, 2015 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-26296469
ABSTRACT
Nanog safeguards pluripotency in mouse embryonic stem cells (mESCs). Insight into the regulation of Nanog is important for a better understanding of the molecular mechanisms that control pluripotency of mESCs. In a silico analysis, we identify four GATA-1 putative binding sites in Nanog proximal promoter. The Nanog promoter activity can be significantly repressed by ectopic expression of GATA-1 evidenced by a promoter reporter assay. Mutation studies reveal that one of the four putative binding sites counts for GATA-1 repressing Nanog promoter activity. Direct binding of GATA-1 on Nanog proximal promoter is confirmed by electrophoretic mobility shift assay and chromatin immunoprecipitation. Our data provide new insights into the expanded regulatory circuitry that coordinates Nanog expression.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Homeodomínio
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Regulação da Expressão Gênica no Desenvolvimento
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Fator de Transcrição GATA1
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2015
Tipo de documento:
Article