Antibacterial Activity and Biosensing of PVA-Lysozyme Microbubbles Formed by Pressurized Gyration.
Langmuir
; 31(36): 9771-80, 2015 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-26307462
ABSTRACT
In this work, the biosensing and antibacterial capabilities of PVA-lysozyme microbubbles have been explored. Gas-filled PVA-lysozyme microbubbles with and without gold nanoparticles in the diameter range of 10 to 250 µm were produced using a single-step pressurized gyration process. Fluorescence microscopy showed the integration of gold nanoparticles on the shell of the microbubbles. Microbubbles prepared with gold nanoparticles showed greater optical extinction values than those without gold nanoparticles, and these values increased with the concentration of the gold nanoparticles. Both types of microbubbles showed antibacterial activity against Gram-negative Escherichia coli (E. coli), with the bubbles containing the gold nanoparticles performing better than the former. The conjugation of the microbubbles with alkaline phosphatase allowed the detection of pesticide paraoxon in aqueous solution, and this demonstrates the biosensing capabilities of these microbubbles.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Álcool de Polivinil
/
Técnicas Biossensoriais
/
Muramidase
/
Antibacterianos
Idioma:
En
Revista:
Langmuir
Assunto da revista:
QUIMICA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Reino Unido