Prevalence of Toxoplasma gondii in Dogs in Zhanjiang, Southern China.
Korean J Parasitol
; 53(4): 493-6, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-26323850
ABSTRACT
Toxoplasmosis, caused by Toxoplasma gondii, is a parasitic zoonosis with worldwide distribution. The present study investigated the prevalence of T. gondii in dogs in Zhanjiang city, southern China, using both serological and molecular detection. A total of 364 serum samples and 432 liver tissue samples were collected from the slaughter house between December 2012 and January 2013 and were examined for T. gondii IgG antibody by ELISA and T. gondii DNA by semi-nested PCR based on B1 gene, respectively. The overall seroprevalence of T. gondii IgG antibody was 51.9%, and T. gondii DNA was detected in 37 of 432 (8.6%) liver tissue samples. These positive DNA samples were analyzed by PCR-RFLP at 3'- and 5'-SAG2. Only 8 samples gave the PCR-RFLP data, and they were all classified as type I, which may suggest that the T. gondii isolates from dogs in Zhanjiang city may represent type I or type I variant. This study revealed the high prevalence of T. gondii infection in dogs in Zhanjiang city, southern China. Integrated measures should be taken to prevent and control toxoplasmosis in dogs in this area for public health concern.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxoplasma
/
Toxoplasmose Animal
/
Doenças do Cão
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Korean J Parasitol
Assunto da revista:
PARASITOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article