PEG-PLA nanoparticles facilitate siRNA knockdown in adult zebrafish heart.
Dev Biol
; 406(2): 196-202, 2015 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-26327645
ABSTRACT
The remarkable regenerative capacity of the zebrafish has made it an important model organism for studying heart regeneration. However, current loss-of-function studies are limited by a lack of conditional-knockout and effective gene-knockdown methods for the adult heart. Here, we report a novel siRNA knockdown method facilitated by poly(ethylene glycol)-b-poly(D,L-lactide) (PEG-PLA) nanoparticles. The siRNA-encapsulated nanoparticles successfully entered cells and resulted in remarkable gene-specific knockdown in the adult heart. This effect was demonstrated by down-regulation of the Aldh1a2 and Dusp6 proteins after intrapleural delivery of nanoparticle-encapsulated siRNAs. Furthermore, siRNA-mediated knockdown of Aldh1a2 was sufficient to inhibit myocardial proliferation and decrease the numbers of Gata4-positive cardiomyocytes after ventricular resection. Therefore, the results of this work demonstrate that nanoparticle-facilitated siRNA delivery provides an alternative tool for loss-of-function studies of genes in the adult heart in particular and other organs in general in the adult zebrafish.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
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Peixe-Zebra
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RNA Interferente Pequeno
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Interferência de RNA
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Nanopartículas
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Técnicas de Silenciamento de Genes
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Miocárdio
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China