Adenylate cyclase of human parathyroid gland.
J Clin Endocrinol Metab
; 47(2): 319-25, 1978 Aug.
Article
em En
| MEDLINE
| ID: mdl-263299
ABSTRACT
Experiments were performed on a particulate fraction from human parathyroid glands. A high activity of adenylate cyclase was detected which was linear with time and protein concentration. The enzyme had an optimum pH in the range of 7-8 and a Km for ATP of 0.44 X 10(-3) M. Ca++ had a profound inhibitory effect; a concentration of 0.5 mM Ca++ reduced enzyme activity by 60%. Maximal enzyme activity was obtained with 5 mM Mg++; higher concentrations of this cation also inhibited enzyme activity. The effect of Mn++ was similar to that of Mg++. Enzyme activity was stimulated by NaF, catecholamines, glucagon, and calcitonin. The effect of catecholamines seems to be mediated through beta-adrenergic receptors.
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Base de dados:
MEDLINE
Assunto principal:
Glândulas Paratireoides
/
Adenilil Ciclases
Limite:
Humans
Idioma:
En
Revista:
J Clin Endocrinol Metab
Ano de publicação:
1978
Tipo de documento:
Article