Development and Application of a Novel SPE-Method for Bioassay-Guided Fractionation of Marine Extracts.
Mar Drugs
; 13(9): 5736-49, 2015 Sep 11.
Article
em En
| MEDLINE
| ID: mdl-26378547
ABSTRACT
The biological diversity of marine habitats is a unique source of chemical compounds with potential use as pharmaceuticals, cosmetics and dietary supplements. However, biological screening and chemical analysis of marine extracts pose specific technical constraints and require adequate sample preparation. Here we report an improved method on Solid Phase Extraction (SPE) to fractionate organic extracts containing high concentration of salt that hampers the recovery of secondary metabolites. The procedure uses a water suspension to load the extracts on a poly(styrene-divynylbenzene)-based support and a stepwise organic solvent elution to effectively desalt and fractionate the organic components. The novel protocol has been tested on MeOH-soluble material from three model organisms (Reniera sarai, Dendrilla membranosa and Amphidinium carterae) and was validated on a small panel of 47 marine samples, including sponges and protists, within discovery programs for identification of immuno-stimulatory and anti-infective natural products.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poríferos
/
Dinoflagellida
/
Extração em Fase Sólida
/
Fracionamento Químico
Limite:
Animals
Idioma:
En
Revista:
Mar Drugs
Assunto da revista:
BIOLOGIA
/
FARMACOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Itália