A strategy for dissecting the architectures of native macromolecular assemblies.
Nat Methods
; 12(12): 1135-8, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26436480
ABSTRACT
It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Conformação Proteica
/
Espectrometria de Massas
/
Modelos Moleculares
/
Complexos Multiproteicos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos