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Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain.
Patel, Jay P; Sowers, Mark L; Herring, Jason L; Theruvathu, Jacob A; Emmett, Mark R; Hawkins, Bridget E; Zhang, Kangling; DeWitt, Douglas S; Prough, Donald S; Sowers, Lawrence C.
Afiliação
  • Patel JP; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Sowers ML; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Herring JL; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Theruvathu JA; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Emmett MR; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Hawkins BE; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Zhang K; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • DeWitt DS; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Prough DS; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
  • Sowers LC; Department of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, Univ
Chem Res Toxicol ; 28(12): 2352-63, 2015 Dec 21.
Article em En | MEDLINE | ID: mdl-26447562
ABSTRACT
The DNA of all organisms is metabolically active due to persistent endogenous DNA damage, repair, and enzyme-mediated base modification pathways important for epigenetic reprogramming and antibody diversity. The free bases released from DNA either spontaneously or by base excision repair pathways constitute DNA metabolites in living tissues. In this study, we have synthesized and characterized the stable-isotope standards for a series of pyrimidines derived from the normal DNA bases by oxidation and deamination. We have used these standards to measure free bases in small molecule extracts from rat brain. Free bases are observed in extracts, consistent with both endogenous DNA damage and 5-methylcytosine demethylation pathways. The most abundant free base observed is uracil, and the potential sources of uracil are discussed. The free bases measured in tissue extracts constitute the end product of DNA metabolism and could be used to reveal metabolic disturbances in human disease.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pirimidinas / Dano ao DNA / Encéfalo / Química Encefálica Limite: Animals Idioma: En Revista: Chem Res Toxicol Assunto da revista: TOXICOLOGIA Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pirimidinas / Dano ao DNA / Encéfalo / Química Encefálica Limite: Animals Idioma: En Revista: Chem Res Toxicol Assunto da revista: TOXICOLOGIA Ano de publicação: 2015 Tipo de documento: Article