Design and efficacy of a single-use bioreactor for heart valve tissue engineering.
J Biomed Mater Res B Appl Biomater
; 105(2): 249-259, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-26469196
ABSTRACT
Heart valve tissue engineering offers the promise of improved treatments for congenital heart disorders; however, widespread clinical availability of a tissue engineered heart valve (TEHV) has been hindered by scientific and regulatory concerns, including the lack of a disposable, bioreactor system for nondestructive valve seeding and mechanical conditioning. Here we report the design for manufacture and the production of full scale, functional prototypes of such a system. To evaluate the efficacy of this bioreactor as a tool for seeding, ovine aortic valves were decellularized and subjected to seeding with human mesenchymal stem cells (hMSC). The effects of pulsatile conditioning using cyclic waveforms tuned to various negative and positive chamber pressures were evaluated, with respect to the seeding of cells on the decellularized leaflet and the infiltration of seeded cells into the interstitium of the leaflet. Infiltration of hMSCs into the aortic valve leaflet was observed following 72 h of conditioning under negative chamber pressure. Additional conditioning under positive pressure improved cellular infiltration, while retaining gene expression within the MSC-valve interstitial cell phenotype lineage. This protocol resulted in a subsurface pilot population of cells, not full tissue recellularization. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B Appl Biomater, 105B 249-259, 2017.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Valva Aórtica
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Bioprótese
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Próteses Valvulares Cardíacas
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Reatores Biológicos
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Engenharia Tecidual
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Células-Tronco Mesenquimais
Tipo de estudo:
Guideline
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Biomed Mater Res B Appl Biomater
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2017
Tipo de documento:
Article