Evidence of Nonuniformity in Urothelium Barrier Function between the Upper Urinary Tract and Bladder.
J Urol
; 195(3): 763-70, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26478446
ABSTRACT
PURPOSE:
We compared the relative permeability of upper urinary tract and bladder urothelium to mitomycin C. MATERIALS ANDMETHODS:
Ex vivo porcine bladder, ureters and kidneys were dissected out and filled with 1 mg ml(-1) mitomycin C. At 60 minutes the organs were emptied and excised tissue samples were sectioned parallel to the urothelium. Sectioned tissue was homogenized and extracted mitomycin C was quantified. Transurothelial permeation across the different urothelia was calculated by normalizing the total amount of drug extracted to the surface area of the tissue sample. Average mitomycin C concentrations at different tissue depths (concentration-depth profiles) were calculated by dividing the total amount of drug recovered by the total weight of tissue.RESULTS:
Mitomycin C permeation across the ureteral urothelium was significantly greater than across the bladder and renal pelvis urothelium (9.07 vs 0.94 and 3.61 µg cm(-2), respectively). Concentrations of mitomycin C in the ureter and kidney were markedly higher than those achieved in the bladder at all tissue depths. Average urothelial mitomycin C concentrations were greater than 6.5-fold higher in the ureter and renal pelvis than in the bladder.CONCLUSIONS:
To our knowledge we report for the first time that the upper urinary tract and bladder show differing permeability to a single drug. Ex vivo porcine ureter is significantly more permeable to mitomycin C than bladder urothelium and consequently higher mitomycin C tissue concentrations can be achieved after topical application. Data in this study correlate with the theory that mammalian upper tract urothelium represents a different cell lineage than that of the bladder and it is innately more permeable to mitomycin C.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ureter
/
Bexiga Urinária
/
Mitomicina
/
Urotélio
Limite:
Animals
Idioma:
En
Revista:
J Urol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Reino Unido