Spatial frequency-domain multiplexed microscopy for simultaneous, single-camera, one-shot, fluorescent, and quantitative-phase imaging.
Opt Lett
; 40(21): 4839-42, 2015 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-26512463
ABSTRACT
Multimodal imaging is a crucial tool when imaging biological phenomena that cannot be comprehensively captured by a single modality. Here, we introduce a theoretical framework for spatial-frequency-multiplexed microscopy via off-axis interference as a novel wide-field imaging technique that enables true simultaneous multimodal and multichannel wide-field imaging. We experimentally demonstrate this technique for single-camera, simultaneous two-channel fluorescence and one-channel quantitative-phase imaging for fluorescent microspheres and fixed cells stained for F-actin and nuclear fluorescence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interpretação de Imagem Assistida por Computador
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Núcleo Celular
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Imagem Molecular
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Imagem Multimodal
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Microscopia de Fluorescência
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Opt Lett
Ano de publicação:
2015
Tipo de documento:
Article