Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis.
Chem Commun (Camb)
; 52(12): 2477-80, 2016 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-26575161
ABSTRACT
We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.
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Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
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Enzimas
Idioma:
En
Revista:
Chem Commun (Camb)
Assunto da revista:
QUIMICA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos