Three-dimensional force microscopy of cells in biopolymer networks.
Nat Methods
; 13(2): 171-6, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26641311
ABSTRACT
We describe a technique for the quantitative measurement of cell-generated forces in highly nonlinear three-dimensional biopolymer networks that mimic the physiological situation of living cells. We computed forces of MDA-MB-231 breast carcinoma cells from the measured network deformations around the cells using a finite-element approach based on a constitutive equation that captures the complex mechanical properties of diverse biopolymers such as collagen gels, fibrin gels and Matrigel. Our measurements show that breast carcinoma cells cultured in collagen gels generated nearly constant forces regardless of the collagen concentration and matrix stiffness. Furthermore, time-lapse force measurements showed that these cells migrated in a gliding motion with alternating phases of high and low contractility, elongation, migratory speed and persistence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biopolímeros
/
Neoplasias da Mama
/
Microscopia de Força Atômica
/
Imageamento Tridimensional
Limite:
Female
/
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Alemanha