In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.
Int Endod J
; 50(1): 48-57, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-26650723
ABSTRACT
AIM:
To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.METHODOLOGY:
A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t-tests and the Mann-Whitney U test were performed.RESULTS:
The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).CONCLUSIONS:
The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Diferenciação Celular
/
Colágeno
/
Alginatos
/
Alicerces Teciduais
/
Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Revista:
Int Endod J
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
França