Structural Basis of Vesicle Formation at the Inner Nuclear Membrane.
Cell
; 163(7): 1692-701, 2015 Dec 17.
Article
em En
| MEDLINE
| ID: mdl-26687357
ABSTRACT
Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear envelope. Cargo is recruited, enveloped at the inner nuclear membrane (INM), and delivered by membrane fusion at the outer nuclear membrane. To understand the structural underpinning for this trafficking, we investigated nuclear egress of progeny herpesvirus capsids where capsid envelopment is mediated by two viral proteins, forming the nuclear egress complex (NEC). Using a multi-modal imaging approach, we visualized the NEC in situ forming coated vesicles of defined size. Cellular electron cryo-tomography revealed a protein layer showing two distinct hexagonal lattices at its membrane-proximal and membrane-distant faces, respectively. NEC coat architecture was determined by combining this information with integrative modeling using small-angle X-ray scattering data. The molecular arrangement of the NEC establishes the basic mechanism for budding and scission of tailored vesicles at the INM.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Capsídeo
/
Vesículas Transportadoras
/
Transporte Ativo do Núcleo Celular
/
Membrana Nuclear
Limite:
Animals
Idioma:
En
Revista:
Cell
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Reino Unido