Estrogen receptor ß controls MMP-19 expression in mouse ovaries during ovulation.
Reproduction
; 151(3): 253-9, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26700939
ABSTRACT
Estrogen receptor beta (ERß/ESR2) has a central role in mouse ovaries, as ERß knockout (BERKO) mice are subfertile due to an increase in fibrosis around the maturing follicle and a decrease in blood supply. This has a consequence that these follicles rarely rupture to release the mature oocyte. Matrix metalloproteinases (MMPs) are modulators of the extracellular matrix, and the expression of one specific MMP, MMP-19, is normally increased in granulosa cells during their maturation until ovulation. In this study, we demonstrate that MMP-19 levels are downregulated in BERKO mouse ovaries. Using human MCF-7 cells that overexpress ERß, we could identify MMP-19 to be a transcriptional target of ligand-bound activated ERß acting on a specificity protein-1 binding site. These data provide a molecular explanation for the observed follicle rupture defect that contributes to the subfertility of female BERKO mice.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ovário
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Receptor beta de Estrogênio
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Metaloproteinases da Matriz Secretadas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
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Humans
Idioma:
En
Revista:
Reproduction
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos