Methylation patterns of Vegfb promoter are associated with gene and protein expression levels: the effects of dietary fatty acids.
Eur J Nutr
; 56(2): 715-726, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-26707994
ABSTRACT
PURPOSE:
We have investigated the epigenetic regulation by dietary fatty acids of Vegfb levels in rats' white adipose tissue and 3T3-L1 cells.METHODS:
A group of rats were assigned to three diets, each one with a different composition of saturated, monounsaturated and polyunsaturated fatty acids. Samples of white adipose tissues were taken for the methylation and expression studies. Additionally, 3T3-L1 cells were treated with palmitic, oleic, and linoleic fatty acids. After treatment, cells were harvested and genetic material was extracted for the analysis of Vegfb levels.RESULTS:
We report evidence of changes in the methylation levels of the CpG island at the Vegfb promoter and in the Vegfb expression levels in vivo and in vitro by dietary fatty acid, with the main contribution of the linoleic fatty acid. Vegfb promoter methylation levels were closely related to the Vegfb gene expression.CONCLUSION:
According to our results, the regulation of Vegfb gene expression by dietary fatty acids may be mediated, at least in part, by epigenetic modifications on Vegfb promoter methylation. Considering the deep association between angiogenesis and tissue growth, we suggest the nutriepigenetic regulation of Vegfb as a key target in the control of the adipose tissue expansion.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Gorduras na Dieta
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Regulação da Expressão Gênica
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Regiões Promotoras Genéticas
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Metilação de DNA
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Fator B de Crescimento do Endotélio Vascular
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Adipócitos Brancos
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Eur J Nutr
Assunto da revista:
CIENCIAS DA NUTRICAO
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Espanha