Mechanism of Archaeal MCM Helicase Recruitment to DNA Replication Origins.
Mol Cell
; 61(2): 287-96, 2016 Jan 21.
Article
em En
| MEDLINE
| ID: mdl-26725007
ABSTRACT
Cellular DNA replication origins direct the recruitment of replicative helicases via the action of initiator proteins belonging to the AAA+ superfamily of ATPases. Archaea have a simplified subset of the eukaryotic DNA replication machinery proteins and possess initiators that appear ancestral to both eukaryotic Orc1 and Cdc6. We have reconstituted origin-dependent recruitment of the homohexameric archaeal MCM in vitro with purified recombinant proteins. Using this system, we reveal that archaeal Orc1-1 fulfills both Orc1 and Cdc6 functions by binding to a replication origin and directly recruiting MCM helicase. We identify the interaction interface between these proteins and reveal how ATP binding by Orc1-1 modulates recruitment of MCM. Additionally, we provide evidence that an open-ring form of the archaeal MCM homohexamer is loaded at origins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sulfolobus
/
DNA Helicases
/
Origem de Replicação
/
Proteínas Arqueais
Idioma:
En
Revista:
Mol Cell
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos