ß-Carotene Induces Apoptosis in Human Esophageal Squamous Cell Carcinoma Cell Lines via the Cav-1/AKT/NF-κB Signaling Pathway.
J Biochem Mol Toxicol
; 30(3): 148-57, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26733226
ABSTRACT
ß-carotene, a type of terpenoid, has many metabolic and physiological functions. In particular, ß-carotene has an antitumor effect. However, the efficacy of ß-carotene against esophageal squamous cell carcinoma (ESCC) remains unclear. In our study, ß-carotene inhibited the growth of ESCC cells and downregulated expression of the Caveolin-1 (Cav-1) protein. Cav-1 protein was expressed only in ESCC cells, not in Het-1A cells. Moreover, ß-carotene triggered apoptosis, induced cell cycle G0/G1 phase arrest, and inhibited cell migration. To explore the mechanism involved in these processes, we further examined the effect of ß-carotene on the Cav-1-mediated AKT/NF-κB pathway. The results showed that the level of AKT and NF-κB phosphorylation was dramatically inhibited, which led to an increase in the Bax/Bcl-2 ratio. Correspondingly, the activity of Caspase-3 was also enhanced. These data suggest that ß-carotene has an antiproliferative role in ESCC cells and may be a promising chemotherapeutic agent for use against ESCC cells.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Esofágicas
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Carcinoma de Células Escamosas
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Transdução de Sinais
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NF-kappa B
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Apoptose
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Beta Caroteno
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Proteínas Proto-Oncogênicas c-akt
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Caveolina 1
Limite:
Humans
Idioma:
En
Revista:
J Biochem Mol Toxicol
Assunto da revista:
BIOLOGIA MOLECULAR
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BIOQUIMICA
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TOXICOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article