Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions.
Cell Stem Cell
; 18(1): 53-65, 2016 Jan 07.
Article
em En
| MEDLINE
| ID: mdl-26748756
ABSTRACT
Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma Humano
/
Técnicas Genéticas
/
Células-Tronco Pluripotentes
/
Sistemas CRISPR-Cas
Limite:
Humans
Idioma:
En
Revista:
Cell Stem Cell
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos