Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta.
Virology
; 490: 27-40, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26808923
ABSTRACT
Plant viruses have evolved to undermine the RNA silencing pathway by expressing suppressor protein(s) that interfere with one or more key components of this antiviral defense. Here we show that the recently identified RNA silencing suppressor (RSS) of lettuce necrotic yellows virus (LNYV), phosphoprotein P, binds to RNA silencing machinery proteins AGO1, AGO2, AGO4, RDR6 and SGS3 in protein-protein interaction assays when transiently expressed. In planta, we demonstrate that LNYV P inhibits miRNA-guided AGO1 cleavage and translational repression, and RDR6/SGS3-dependent amplification of silencing. Analysis of LNYV P deletion mutants identified a C-terminal protein domain essential for both local RNA silencing suppression and interaction with AGO1, AGO2, AGO4, RDR6 and SGS3. In contrast to other viral RSS known to disrupt AGO activity, LNYV P sequence does not contain any recognizable GW/WG or F-box motifs. This suggests that LNYV P may represent a new class of AGO binding proteins.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Proteínas de Plantas
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Rhabdoviridae
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Nicotiana
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Proteínas Virais
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Interferência de RNA
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Proteínas Argonautas
Idioma:
En
Revista:
Virology
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Austrália