Electrochemical detection of Pseudomonas in wound exudate samples from patients with chronic wounds.
Wound Repair Regen
; 24(2): 366-72, 2016 03.
Article
em En
| MEDLINE
| ID: mdl-26815644
ABSTRACT
In clinical practice, point-of-care diagnostic testing has progressed rapidly in the last decade. For the field of wound care, there is a compelling need to develop rapid alternatives for bacterial identification in the clinical setting, where it generally takes over 24 hours to receive a positive identification. Even new molecular and biochemical identification methods require an initial incubation period of several hours to obtain a sufficient number of cells prior to performing the analysis. Here we report the use of an inexpensive, disposable electrochemical sensor to detect pyocyanin, a unique, redox-active quorum sensing molecule released by Pseudomonas aeruginosa, in wound fluid from patients with chronic wounds enrolled in the WE-HEAL Study. By measuring the metabolite excreted by the cells, this electrochemical detection strategy eliminates sample preparation, takes less than a minute to complete, and requires only 7.5 µL of sample to complete the analysis. The electrochemical results were compared against 16S rRNA profiling using 454 pyrosequencing. Blind identification yielded 9 correct matches, 2 false negatives, and 3 false positives giving a sensitivity of 71% and specificity of 57% for detection of Pseudomonas. Ongoing enhancement and development of this approach with a view to develop a rapid point-of-care diagnostic tool is planned.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Pseudomonas
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Infecção dos Ferimentos
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Técnicas Biossensoriais
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Sistemas Automatizados de Assistência Junto ao Leito
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Exsudatos e Transudatos
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Técnicas Eletroquímicas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adult
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Female
/
Humans
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Male
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Middle aged
Idioma:
En
Revista:
Wound Repair Regen
Assunto da revista:
DERMATOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article