Biodegradable Inorganic Nanovector: Passive versus Active Tumor Targeting in siRNA Transportation.
Angew Chem Int Ed Engl
; 55(14): 4582-6, 2016 Mar 24.
Article
em En
| MEDLINE
| ID: mdl-26879376
ABSTRACT
The biodegradable inorganic nanovector based on a layered double hydroxide (LDH) holds great promise for gene and drug delivery systems. However, inâ
vivo targeted delivery of genes through LDH still remains a key challenge in the development of RNA interference therapeutics. Here, we describe inâ
vivo and inâ
vitro delivery system for Survivin siRNA (siSurvivin) assembled with passive LDH with a particle size of 100â
nm or active LDH conjugated with a cancer overexpressing receptor targeting ligand, folic acid (LDHFA), conferring them an ability to target the tumor by either EPR-based clathrin-mediated or folate receptor-mediated endocytosis. When not only transfected into KB cells but also injected into xenograft mice, LDHFA/siSurvivin induced potent gene silencing at mRNA and protein levels inâ
vitro, and consequently achieved a 3.0-fold higher suppression of tumor volume than LDH/siSurvivin inâ
vivo. This anti-tumor effect was attributed to a selectively 1.2-fold higher accumulation of siSurvivin in tumor tissue compared with other organs. Targeting to the tumor with inorganic nanovector can guide and accelerate an evolution of next-generation theranosis system.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
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RNA Interferente Pequeno
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Nanoestruturas
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Vetores Genéticos
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Neoplasias
Limite:
Animals
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2016
Tipo de documento:
Article