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Unique Expression of Angiotensin Type-2 Receptor in Sex-Specific Distribution of Myelinated Ah-Type Baroreceptor Neuron Contributing to Sex-Dimorphic Neurocontrol of Circulation.
Liu, Yang; Zhou, Jia-Ying; Zhou, Yu-Hong; Wu, Di; He, Jian-Li; Han, Li-Min; Liang, Xiao-Bo; Wang, Lu-Qi; Lu, Xiao-Long; Chen, Hanying; Qiao, Guo-Fen; Shou, Weinian; Li, Bai-Yan.
Afiliação
  • Liu Y; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Zhou JY; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Zhou YH; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Wu D; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • He JL; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Han LM; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Liang XB; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Wang LQ; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Lu XL; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Chen H; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Qiao GF; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Shou W; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
  • Li BY; From the Department of Pharmacology (Y.L., J.-Y.Z., Y.-H.Z., D.W., J.-L.H., L.-M.H., X.-B.L., L.-Q.W., X.-L.L., G.-F.Q., B.-Y.L.) and Key Laboratory of Cardiovascular Medicine Research of Ministry of Education (Y.-H.Z., D.W., L.-Q.W., X,-B.L., G.-F.Q.), Harbin Medical University, Harbin, Heilongjian
Hypertension ; 67(4): 783-91, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26883269
ABSTRACT
This study aims to understand the special expression patterns of angiotensin-II receptor (AT1R and AT2R) in nodose ganglia and nucleus of tractus solitary of baroreflex afferent pathway and their contribution in sex difference of neurocontrol of blood pressure regulation. In this regard, action potentials were recorded in baroreceptor neurons (BRNs) using whole-cell patch techniques; mRNA and protein expression of AT1R and AT2R in nodose ganglia and nucleus of tractus solitary were evaluated using real time-polymerase chain reaction, Western blot, and immunohistochemistry at both tissue and single-cell levels. The in vivo effects of 17ß-estradiol on blood pressure and AT2R expression were also tested. The data showed that AT2R, rather than AT1R, expression was higher in female than age-matched male rats. Moreover, AT2R was downregulated in ovariectomized rats, which was restored by the administration of 17ß-estradiol. Single-cell real time-polymerase chain reaction data indicated that AT2R was uniquely expressed in Ah-type BRNs. Functional study showed that long-term administration of 17ß-estradiol significantly alleviated the blood pressure increase in ovariectomized rats. Electrophysiological recordings showed that angiotensin-II treatment increased the neuroexcitability more in Ah- than C-type BRNs, whereas no such effect was observed in A-types. In addition, angiotensin-II treatment prolonged action potential duration, which was not further changed by iberiotoxin. The density of angiotensin-II-sensitive K(+) currents recorded in Ah-types was equivalent with iberiotoxin-sensitive component. In summary, the unique, sex- and afferent-specific expression of AT2R was identified in Ah-type BRNs, and AT2R-mediated KCa1.1 inhibition in Ah-type BRNs may exert great impacts on baroreflex afferent function and blood pressure regulation in females.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pressorreceptores / Pressão Sanguínea / Angiotensina II / Barorreflexo / Receptor Tipo 2 de Angiotensina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Hypertension Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pressorreceptores / Pressão Sanguínea / Angiotensina II / Barorreflexo / Receptor Tipo 2 de Angiotensina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Hypertension Ano de publicação: 2016 Tipo de documento: Article