Condensin Smc2-Smc4 Dimers Are Flexible and Dynamic.
Cell Rep
; 14(8): 1813-8, 2016 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-26904946
ABSTRACT
Structural maintenance of chromosomes (SMC) protein complexes, including cohesin and condensin, play key roles in the regulation of higher-order chromosome organization. Even though SMC proteins are thought to mechanistically determine the function of the complexes, their native conformations and dynamics have remained unclear. Here, we probe the topology of Smc2-Smc4 dimers of the S. cerevisiae condensin complex with high-speed atomic force microscopy (AFM) in liquid. We show that the Smc2-Smc4 coiled coils are highly flexible polymers with a persistence length of only â¼ 4 nm. Moreover, we demonstrate that the SMC dimers can adopt various architectures that interconvert dynamically over time, and we find that the SMC head domains engage not only with each other, but also with the hinge domain situated at the other end of the â¼ 45-nm-long coiled coil. Our findings reveal structural properties that provide insights into the molecular mechanics of condensin complexes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas Nucleares
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Proteínas Cromossômicas não Histona
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Proteínas de Transporte
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Cromossomos Fúngicos
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Proteínas de Saccharomyces cerevisiae
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Multimerização Proteica
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Revista:
Cell Rep
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Holanda