Delivery of Therapeutic Proteins via Extracellular Vesicles: Review and Potential Treatments for Parkinson's Disease, Glioma, and Schwannoma.
Cell Mol Neurobiol
; 36(3): 417-27, 2016 Apr.
Article
em En
| MEDLINE
| ID: mdl-27017608
ABSTRACT
Extracellular vesicles present an attractive delivery vehicle for therapeutic proteins. They intrinsically contain many proteins which can provide information to other cells. Advantages include reduced immune reactivity, especially if derived from the same host, stability in biologic fluids, and ability to target uptake. Those from mesenchymal stem cells appear to be intrinsically therapeutic, while those from cancer cells promote tumor progression. Therapeutic proteins can be loaded into vesicles by overexpression in the donor cell, with oligomerization and membrane sequences increasing their loading. Examples of protein delivery for therapeutic benefit in pre-clinical models include delivery of catalase for Parkinson's disease to reduce oxidative stress and thus help neurons to survive; prodrug activating enzymes which can convert a prodrug which crosses the blood-brain barrier into a toxic chemotherapeutic drug for schwannomas and gliomas; and the apoptosis-inducing enzyme, caspase-1 under a Schwann cell specific promoter for schwannoma. This therapeutic delivery strategy is novel and being explored for a number of diseases.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doença de Parkinson
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Neoplasias Encefálicas
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Proteínas Recombinantes
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Sistemas de Liberação de Medicamentos
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Vesículas Extracelulares
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Glioma
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Neurilemoma
Limite:
Animals
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Humans
Idioma:
En
Revista:
Cell Mol Neurobiol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos